Cloning, sequencing, and overexpression of the Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (pckA) gene

Article Abstract:

The phosphoenolpyruvate (PEP) carboxykinase-encoding gene from the anaerobic, CO2-fixing, succinate-producing bacterium Anaerobiospirillum succiniciproducens was cloned, sequenced and expressed in Escherichia coli. The gene encoded a 532-amino acid polypeptide with a molecular mass of 58.7 kDa. The sequence of the A. succiniciproducens PEP carboxykinase was similar to those of all known ATP/ADP-dependent PEP carboxykinases.

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Insights into Actinobacillus succinogenes fermentative metabolism in a chemically defined growth medium

Article Abstract:

A defined medium, AM3, is created to expand the experimental opportunities for investigating the fermentative metabolism of succinate-producing Actinobacillus succinogenes. AM3 is a phosphate-buffered medium containing vitamins, minerals, NH4Cl as the main nitrogen source, and glutamate, cysteine, and methionine as required amino acids.

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Effect of overexpression of actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli

Article Abstract:

Succinate fermentation was investigated in Escherichia coli strains over expressing actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). Results showed that PEPCK overexpression increased succinate production 6.5- fold.

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