Article Abstract:
The genes encoding phosphotransacetylase (PTA) and acetate kinase (AK) from Clostridium acetobutylicum are cloned and sequenced to find the features of these enzymes. The AK and PTA show elevated activities on cell extracts from Escherichia coli and C. acetobutylicum harboring the subclone. The pta and ack genes lie near the pta upstream in the clostridial chromosome. While pta gene encodes a protein of 333 amino acid residues, ack encodes a polypeptide of 401 residues. The pta gene has an operon arrangement with a single transcriptional start site lying 70 bp upstream of the start codon.
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Article Abstract:
Non-replicative integrational plasmids containing butyrate kinase (buk) and phosphotransacetylase (pta) gene fragments were used to inactivate buk and pta in Clostridium acetobutylicum ATCC 824. Inactivation of the pta gene on the chromosome reduced pta and acetate kinase activity as well as acetate production, while inactivation of the buk gene minimized buk activity and butyrate production but increased butanol production.
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Article Abstract:
A single homologue to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824 are identified. A demonstration that the promoters of abrB1941 and abrB3646 were not active under the growth conditions tested using reporter vectors is presented.
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