Article Abstract:
The recombinant strain constructed by the cloning of the genes for L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH) from Gluconobacter oxydans T-100, produces 2-keto-L-gulonate (2-KLGA) from D-sorbitol. The high level production of 2-KLGA, a precursor of L-ascorbic acid is done by simple fermentation of the recombinant Gluconobacter. DNA sequencing show that the SDH and SNDH are coded in sequential open reading frames with 1,497 and 1,599 nucleotides respectively. The DNA is used to obtain an expression vector, pSDH155, which produces 2-KLGA from D-sorbitol in G. oxydans G624.
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Article Abstract:
A bacterial strain that can grow on cyclohexylamine has been synthesized using enrichment and isolation methods. The strain, IH-35A, belongs to the genus Brevibacterium oxydans and induces the cyclohexylamine oxidase, cyclohexanone monooxygenase and 6-hexanolactone hydrolase activities by growth on cyclohexylamine. Cyclohexylamine degradation is induced by cyclohexanone, 6-hexanolactone, 6-hydroxyhexanoate and adipate.
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Article Abstract:
A study conducted showed that a bacterium isolated from patulin-contaminated apples was capable of degrading patulin to a less-toxic compound ascladiol. The observations showed degradation up to 96% of patulin in apple juices containing up to 800 [mu]g/ml of patulin and incubated with Gluconobacter oxydans.
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