Article Abstract:
The gene encoding citrate synthase from Bacillus sp. strain C4 was isolated and sequenced. The compelete nucleleotide sequence for the entire 3.1-kb HindIII fragment was derived revealing one major open reading frame which coding for citrate synthase, ctsA. The citrate synthase isolated from the strain C4 was a dimer with a subunit of Mr of 42, 000. The N-terminal amino acid sequence was found to be identifical with the predicted gene sequnce. The amino acid sequence was also compared with citrate synthase secreted by other gram-negative bacteria.
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Article Abstract:
The first genetic transformation system, shuttle vectors and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus are presented. Two low-copy-number Escherichia coli-B. methanolicus shuttle plasmids, pDQ507 and pDQ508 are shown. pDQ508 contains the replication origin cloned from a 17-kb endogenous B. methanolicus plasmid, pBM1. pDQ507 contains a cloned B. methanolicus DNA fragment, pmr-1, possibly of chromosomal origin, that supports maintenance of pDQ507 as a circular, extrachromosomal DNA molecule.
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Article Abstract:
Results indicate that Bacillus methanolicus exhibit several forms of citrae synthase and glutamate secretion can be altered to increase L-lysine secretion by regulaing citrate synthase activity. In the citrate synthase deficient-mutant , the level of of glutamate secreted is reduced 7-fold and the ratio of L-lysine to glutamate secreted is enhance 4-5-fold as compared to the wild type.
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