Article Abstract:
A gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was isolated and characterized. Sequence analysis showed that the MGA3 aspartokinase II is a tetramer composed of alpha and beta dimers, having a total mass of 122,000 Da. Homology comparisons showed that the MGA3 aspartokinase is 76% identical to the B. subtilis aspartokinase II. In addition, the analysis of a 300-nucleotide intervening sequence showed that the enzyme may be regulated by a transcription attenuating mechanism similar to that proposed for the B. subtilis tryptophan operon.
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Article Abstract:
The gene encoding citrate synthase from Bacillus sp. strain C4 was isolated and sequenced. The compelete nucleleotide sequence for the entire 3.1-kb HindIII fragment was derived revealing one major open reading frame which coding for citrate synthase, ctsA. The citrate synthase isolated from the strain C4 was a dimer with a subunit of Mr of 42, 000. The N-terminal amino acid sequence was found to be identifical with the predicted gene sequnce. The amino acid sequence was also compared with citrate synthase secreted by other gram-negative bacteria.
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Article Abstract:
The restriction endonuclease Bacillus methanolicus (B. methanolicus) T1 (BmeT1) has been isolated and characterized. BmeT1 is an isoschizomer of BclI that recognizes the DNA sequence 5' TGATCA 3'. Data suggested the existence of a subset of methylate Sau3AI (GATC) sites and a second subset of nonmethylated Sau3AI sites within the B. methanolicus chromosome. Data can be used to develop strategies for inhibiting BmeT1 restriction of DNA introduced into B. methanolicus.
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