Article Abstract:
The use of gyrB genes encoding the subunit B protein of DNA gyrase as targets of highly specific probes was explored in an effort to develop a rapid and reliable approach for differentiating Bacillus cereus from B thuringiensis. To this end, 1.2-kb fragments of the gyrB genes of B cereus, B anthracis, B. thuringiensis and B. mycoides were amplified, cloned and sequenced. A procedure for identifying the target organism in boiled rice regardless of its phenotypes, serotypes and virulence factors succeeded in detecting 0.24 CFU of B cereus cells per gram of boiled rice without extracting DNA.
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Article Abstract:
The pathogenic characteristics of Bacillus thuringiensis and Bacillus cereus were analyzed by the cloning and amino acid sequencing of toxin genes from Bacillus cereus FM1 strain and Bacillus thuringiensis israelensis. Analysis of the enterotoxin genes from the two Bacillus species indicated the presence of beta structures and consecutive Asn residues. Furthermore, the nucleotide sequences of the enterotoxin genes are similar in both Bacillus thuringiensis and Bacillus cereus.
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Article Abstract:
The sequence diversity of the Bacillus thurinigiensis flagellin (H antigen [Hag]) protein is analyzed and compared with H serotype diversity along with other Bacillus ceres sensu lato species and strains. The amplification, cloning and determination of the nucleotide sequences of the flagellin allels from B. thuringiensis H serotypes and from B. thuringiensis-related species was reported in the Bacillus cereus senso lacto group.
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