Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

Article Abstract:

An endo-1,3-1,4-beta-glucanase gene from Bacillus macerans has been cloned and expressed in Lactobacillus reuteri. The lactobacilli secreted the beta-glucanase gene which means that B. macerans secretion signals can be recognized by Lactobacillus cells. The beta-glucanase gene could be used to synthesize heterologous peptides of L. reuteri. A new promoter probe vector for L. reuteri can be made from the synthesis of plasmid pNCKH113.

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Identification of Lactobacillus sakei genes induced during meat fermentation and their role in survival and growth

Article Abstract:

In vivo expression technology (IVET) is used for investigating gene expression of Lactobacillus sakei 23K during meat fermentation. A molecular basis is established that has allowed the investigation of the bacterial properties contributing to the ecological performance of the organism and also influencing the final outcome of sausage fermentation.

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Ecological behavior of Lactobacillus reuteri 100-23 is affected by mutation of the luxS gene

Article Abstract:

The physiological and ecological role for the luxS gene in Lactobacillus reuteri 100-23C is defined by constructing a luxS mutant by insertional mutagenesis. The results show that LuxS influences important ecological attributes of L. reuteri 100-23C, the consequences of which are niche specific.

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