DNA from uncultured organisms as a source of 2,5-diketo-D-gluconic acid reductases

Article Abstract:

Research has been conducted on the environmental DNA. The method of obtaining full-length, functional genes from environmental DNA is described.

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Transformation of fatty acids catalyzed by cytochrome P450 monooxygenase enzymes of Candida tropicalis

Article Abstract:

Results show that the ability to oxidize fatty acids by Candida tropicalis ATCC 20336 is associated with the two cytochrome P450s, CYP52A13 and CYP52A17. Data indicate that both oxidize oleic acid, the former preferentially oxidizes it and unsaturated acids to omega-hydroxy acids, while the latter in addition, convert shorter, saturated fatty acids. Both P450s further oxidize omega-hydroxy fatty acids to alpha,omega-diacids.

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Cloning and characterization of three fatty alcohol oxidase genes from Candida tropicalis strain ATCC 20336

Article Abstract:

Candida tropicalis (ATCC 20336) converts fatty acids to long-chain dicarboxylic acids via a pathway that includes among other reactions the oxidation of omega-hydroxy fatty acids to omega-aldehydes by a fatty alcohol oxidase (FAO). Due to the distinctive differences in the specificities of the two enzymes, analysis of the activities present in C. tropicalis strain H5343 grown on fatty acids revealed that one of the two genes, FAO1, was the major FAO expressed during production of dicarboxylic acids.

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