Article Abstract:
Simple sequence repeats (SSR), or microsatellite loci, have been use for parentage analysis, genetic mapping and studies of population structure but the development of novel loci is difficult and time consuming. A new study attempts to clone di-, tri-, and tetra-nucleotide repeat microsatellites for use in studying the population genetics of two wild plant species, Heliconia acuminata and Corythophora alta. The study shows that the numbers and repeat types of possible SSR markers are dependent upon the restriction endonuclease used to sample the genome during construction of DNA libraries. Details of the study are presented.
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Article Abstract:
Eastern lowland gorillas (Gorilla gorilla graueri) are not well studied, particularly on a molecular level. The internal region of the mitochondrial DNA cytochrome oxidase subunit II (COII) areas and a hypervariable section of the mitochondrial DNA control region (D-loop) from wild lowland gorillas in montane and lowland habitats of Kahuzi-Biega National Park, were sequenced. The COII regions were found to be identical, suggesting previous diagnostic sites may be valid. There was low variability in the D-loop and haplotype frequencies were different in the two habits, indicating demographic distinctions.
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Article Abstract:
A program was developed for the estimation of restriction endonuclease site positions from restriction fragment size and number. The program uses the rapid calculation power of the personal computer to facilitate the deductive decisions needed to construct a circular restriction map using the double digestion technique. Enzymes are utilized to initiate the mapping procedure and the program constructs the site map by orienting single enzyme site maps according to the number and size of the fragments produced by double digests.
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