Article Abstract:
The strain of the Noti- and Sfil-digested genome of Vibrio Cholerae 0139 isolated from different epidemic regions of India reveals clonal origin and the genome size about 2.2 Mb. Examination of the electrophoretic profiles of the genome of these strains, the RFLP of the cholera toxin gene and Southern blot hybridization of Noti-linking clone of classical strain 569B suggest that these strains have high resemble to V. cholerae 01 biotype EI Tor but differ from the classical 01 vibrios. Genetic heterogeneity in the population is demonstrated by the organization of the virulence gene cassettes in different 0139 strains.
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Article Abstract:
Vibrio cholerae belonging to serovars O1 and non-O1, which are biochemically and gentically indistinguishable, differ in cell surface fragility, with O1 being unable to survive environmental fluctuations. An investigation was conducted to determinethe cell surface architecture of non-O1 vibrios. The results showed that non-O1vibrios are more resistant to hydrophobic compounds, detergents and lysis in hypotonic solutions, as compared to O1 isolates. In addition, non-O1 vibrios donot have phospholipids in the outer leaflet of the outer membrane.
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Article Abstract:
Vibrio cholerae cells shifted from 37 degrees Celsius to various low temperatures when it was exponentially grown, revealing that the organism could adapt and grow at temperatures down o 15 degrees Celsius, below which the growth was completely arrested. Analyses of proteins of cold-exposed cells revealed significant induction of two major cold shock proteins (Csps), whose molecular masses were 7.7 kDa and 7.5 kDa and six other Csps, most of which were much larger.
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