Chemoselective nitro group and reductive dechlorination initiate degradation of 2-chloro-5-nitrophenol by Ralstonia eutropha JMP134

Article Abstract:

Cometabolic transformations were found in most studies involving the degradation of chloronitroarenes. In Ralstonia eutropha, its JMP134 was discovered by researchers to employ 2-chloro-5-nitrophenol as a major source of nitrogen, carbon and energy during the initial steps of degradation. Research on the reduction and transformation of reduction products of certain compounds in eukaryotic systems revealed the presence of cytotoxic and mutagenic effects.

author: Spain, Jim C., Lenke, Hiltrud, Knackmuss, Hans-Joachim, Schenzle, Andreas
Physiological aspects, Bacteria, Microbiological synthesis, Microbial biotechnology

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Whole-cell kinetics of trichloroethylene degradation by phenol hydroxylase in Ralstonia eutropha JMP134 derivative

Article Abstract:

Whole-cell kinetics of trichloroethylene (TCE) degradation by suspended batch cultures in Ralstonia eutropha having the recombinant plasmid pYK3021 was examined. Findings showed sustained linear rates of TCE degradation indicating that solvent effects are limited and is only slightly affected by the production of toxic intermediates. Results revealed that Ralstonia eutropha AEK301/pYK3021 is a potential candidate for in situ remediation investigations.

author: Harker, Alan R., Ayoubi, Patricia J.
Methods, Usage, Microbial enzymes, Hydroxylases, Decontamination (from gases, chemicals, etc.), Decontamination

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Trichloroethylene biodegradation by mesophilic and psychrophylic ammonia oxidizers and methanotrophs in groundwater microcosms

Article Abstract:

A study was conducted on the trichloroethylene (TCE) biodegradation stimulation by methane and ammonium at psychrophilic temperatures in groundwater microcosms. Ammonia oxidizers promoted mineralization reduction by 12% and methanotrophs promoted a 5% decrease. Results demonstrate the feasibility of in situ TCE bioremediation at psychrophilic temperatures typical in northern US surficial aquifiers.

author: Hickey, W.J., Moran, B.N.
Aquatic microbiology, Water, Underground, Groundwater

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subjects list: Research, Biodegradation, Trichloroethylene
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