Article Abstract:
Two extraction procedures were used to study the yield and properties of acid-precipitable polymeric lignin (APPL) produced by four strains of Streptomycetes during growth in solid-state fermentation. APPL showed different properties when extracted by water and sodium hydroxide (NaOH). NaOH extraction cleaved ester links between hemicellulose and between hemicellulose and cinnamic acid. Water, however, did not alter the links, therfore allowed a better study of the process of solubilization by the streptomycetes.
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Article Abstract:
Five spontaneous mutants of Streptomyces lividans TK64 resistant to ethidium bromide (EB) were isolated and the corresponding mutations were mapped to two different chromosomal locations. The mutations caused unselected resistance to various basic dyes and norfloxacin. The low-level resistance mutants showed wild-type levels of EB uptake and energy-dependent efflux while the high-level resistance mutants had a reduced accumulation and an increased efflux of EB.
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Article Abstract:
A study was conducted to examine the functions of Streptomyces coelicolor genes in the master control systems for differentiation. Reverse genetics is carried out to produce mutants. The sequences of the early whi genes were not correlated with phosphorelay production in S. coelicolor. Results indicated genes having cell biology and metabolism functions. Findings also showed that the FtsZ protein supports a critical role in bacterial cell division.
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