Article Abstract:
Two autoreplicative regions of the incompatibility group HI2 (IncHI2) plasmid R478 have been characterized, the first autoreplicative regions from an IncHI2 subgroup plasmid to be described. The regions are RepHI2A and RepHI1A(sub.R478). The prototype IncHI2 subgroup plasmid, R478, has been shown to have at least two iteron-controlled autoreplicative regions. The presence of RepHI1A(sub.R478) on R478 likely explains the incompatibility seen between R478 and plasmids of the IncHI1 subgroup. Plasmids in the IncH encode resistance determinants and have been associated with many Salmonella-related epidemics and pandemics.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
The complete nucleotide sequence of a plasmid, pKJ50, extracted from an intestinal bacterium, Bifidobacterium longum KJ, was determined. The plasmid was observed to be 4960 bp in size with a G+C content of 61.7 mol%. Computer analysis of sequence data indicated three major ORFs encoding putative proteins of 31.5, 24.5 and 38.6 kDa. In vitro translation revealed protein bands of the expected sizes corresponding to each ORF. A shuttle vector which can convert Bifidobacterium animalis MB209 was formed by cloning pKJ50 and a chloramphenicol resistance gene into pBR322.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
The identification of the alkene monooxygenase in Rhodococcus corallinus B-276 as the trichloroethene (TCE)-oxidizing enzyme system. TCE degradation was observed to be inducer-independent but growth-phase-dependent. Southern blot analysis with the alkene monooxygenase showed positive signals with linear plasmids of two propene-oxidizing R. ruber strains. The identification of linear plasmids in R. corallinus B-276 provides clear evidence that the alkene monooxygenase is encoded by the linear plasmid pNC30.
User Contributions:
Comment about this article or add new information about this topic: