adhA in Aspergillus parasiticus is involved in conversion of 5'-hydroxyaverantin to averufin

Article Abstract:

Researchers have isolated a gene in Aspergillus parasiticus whose protein product dehydrogenates 5'-hydroxyaverantin to averufin. This is one of the steps in the synthesis of aflatoxin B(sub 1).

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Clustered pathway genes in aflatoxin biosynthesis

Article Abstract:

Research has demonstrated that 25 identified genes clustered within a 70-kb DNA region in the chromosome are involved in aflatoxin biosynthesis. These genes and their enzymes involved in aflatoxin biosynthetic pathway are reviewed and a new naming scheme that follows the naming convention in Aspergillus is proposed.

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Characterization of the critical amino acids of an Aspergillus parasiticus Cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2

Article Abstract:

A study was conducted to clone the ordA and ordA1 genes of the aflatoxigenic strain Aspergillus parasiticus SRRC 143 and SRRC 2043, respectively, and to evaluate the function of the ordA gene in the formation of aflatoxins. Point mutations were introduced into the ordA gene using polymerase chain reaction-based site-directed mutagenesis while mycelia were obtained by filtration. Results indicated that cytochrome P-450 type enzymes were involved in the synthesis of different primary and secondary metabolites in filamentous fungi.

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