Characterization of rhizosphere colonization by luminescent Enterobacter cloacae at the population and single-cell levels

Article Abstract:

The colonization of the rhizosphere of wheat by Enterobacter cloacae was characterized by examing the light output of an E. cloacae strain expressing the luxAB bioluminescence genes. Luminometry revealed an increase in the metabolic activity of E. cloacae in rhizosphere vs. non-rhizosphere soil. Restriction of lux-marked bacteria to the spermosphere was seen in nonsterile soil, indicating that indigenous soil organisms compete with E. cloacae in rhizosphere colonization.

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Reduction of selenium oxyanions by Enterobacter cloacae SLD1a-1: isolation and growth of the bacterium and its expulsion of selenium particles

Article Abstract:

The SLD1a-1 strain of bacterium Enterobacter cloacae has the potential to remove selenium oxyanions from agricultural irrigation water. The bacterium could generate high densities of cells during aerobic growth that could be easily cultured prior to its introduction into the water. E. cloacae could also be used to pretreat contaminated water together with microphytic organisms in integrated remediation systems.

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Design and application of a biosensor for monitoring toxicity of compounds to eukaryotes

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Researchers modified the yeast Saccharomyces cerevisiae to express firefly luciferase whenever it was in the presence of a toxic chemical. It was more sensitive to pollutants than prokaryotic biosensors and its cell wall allows it to survive in extreme conditions.

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