Characterization of plasmid-encoded citrate permease (citP) genes from Leuconostoc species reveals high sequence conservation with the Lactococcus lactis citP gene

Article Abstract:

The citrate permease (citP) gene appears to be plasmid encoded in Leuconostoc species and is highly homologous to the citP gene of Lactococcus lactis subsp. lactis biovar diacetylactis NCDO176. Citrate transport is required for the bacterial production of diacetyl and CO2 from citrate in cheese products. Homology with the L. lactis citP gene allowed the cloning of this gene from several Leuconostoc strains.

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The lactose transporter in Leuconostoc lactis is a new member of the lacS subfamily of galactoside-pentose-hexuronide translocators

Article Abstract:

The Leuconostoc lactis lacS gene and its surrounding DNA regions are cloned by functional complementation of lactose permease-deficient Escherichia coli mutants and subjected to nucleotide sequence analyses for characterization. Results show lactose transport in L. lactis is mediated by a lactose protein similar to lacS proteins from lactic acid bacteria which lack the galactose moiety of lactose.

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Molecular characterization of the restriction endonuclease gene (scrFIR) associated with the ScrFI restriction/modification system from Lactococcus lactis subsp. cremoris UC503

Article Abstract:

The completed nucleotide sequence of the chromosomally encode type II ScrFI restriction/modification system form Lactococcus lactis subsp. cremoris UC503 was reported. The restriction gene was located between the two MTase genes. The deduced amino acid sequence of the ScrFI ENase was found significantly different from other type II ENases, including isoschizomeric enzymes.

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