Does enterohemorrhagic Escherichia coli O157:H7 enter the viable but nonculturable state in salted salmon roe?

Article Abstract:

The high salt content of some foods may not be enough to kill many pathogenic bacteria. Four outbreaks of E. coli O157:H7 infection affecting 62 people occurred in Japan in 1998. All were traced to salted salmon roe. Researchers show that the bacterium can enter a viable but nonculturable state during stressful conditions and reactivate when the stress ends.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

A new method for direct detection of Listeria monocytogenes from foods by PCR

Article Abstract:

A method of preparing DNA from food sources, using alcohol precipitation in a solution of NaI, may be effective in PCR-based testing for contamination with Listeria monocytogenes. When compared with two other methods of DNA extraction from soft cheese and minced meat, this method produced more detectable DNA for PCR analysis. This method could detect as little as 1,000 CFU bacteria per 0.5 grams of food sample.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Characterization of haemagglutinin activity of Clostridium bolulinum type C and D 165 toxins, and one subcomponent of haemagglutinin (HA1)

Article Abstract:

Hemagglutinin activity of Clostridium bolulinum type C and D 165 toxins is discussed with attention to one subcomponent of hemagglutinin (HA1). A new, simple method for purifying type D 165 toxin is presented. The ways in which toxins bind to erythrocytes were investigated using purified toxins.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: