Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis

Article Abstract:

Polymerase chain reaction (PCR) analysis is suggested for detecting genes encoding enterotoxins Bacillus cereus and B. thuringiensis, which cause gastrointestinal diseases including diarrhea. PCR analysis, using primer sets for detecting genes BCET1, BCET3, and BCET4, and protein complexes hemolysin BL and nonhemolytic enterotoxin, proved successful.

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Characterization of emetic Bacillus weihenstephanensis, a new cereulide-producing bacterium

Article Abstract:

An objective is presented of investigating the occurrence of the Bacillus cereus group, to characterize the cereulide producers with regard to psychrotolerance and compare them to popular cereulide producers at the pheno- and genotypic levels. Two psychrotolerant Bacillus weihenstephanensis strains, MC67 and MC118 producing cereulide are reported.

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A rapid PCR-based DNA test for enterotoxic Bacillus cereus

Article Abstract:

The presence of the hblA and bceT hemolysin genes in different strains of Bacillus was determined and correlated with the enterotoxicity in an effort to develop a rapid diagnostic tool to identify Bacillus cereus and determine its enterotoxic potential accurately. Results reveal that the polymerase chain reaction method of screening for the hemolysin HblA complex is an effective and accurate diagnostic tool.

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