Characterization of an H2-utilizing enrichment culture that reductively dechlorinates tetrachloroethene to vinyl chloride and ethene in the absence of methanogenesis and acetogenesis

Article Abstract:

The microbes which dechlorinate tetrachloroethene (or perchloroethylene, PCE) depend on other organisms for their nutrition, as other anaerobes do. An anaerobic H2-PCE enrichment culture, derived from a diluted methanol-PCE culture, dechlorinated PCE to vinyl chloride and ethene. Dechlorination in the presence of H2 requires neither methanol nor acetate as the electron donor and does not produce methane or methanol indicating that methanogenic and acetogenic bacteria are not present. PCE is a major ground water pollutant and it is not degraded by aerobes.

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Hydrogen as an electron donor for dechlorination of tetrachloroethene by an anaerobic mixed culture

Article Abstract:

Previous investigations have shown that a methanogenic enrichment culture can dechlorinate high concentrations of tetrachloroethene (PCE) in a PCE-methanol (MeOH) enrichment culture. This high-PCE enrichment culture was further characterized by comparing results fromMeOH- and hydrogen-fed mixed anaerobic cultures amended with selective inhibitors of eubacteria and methanogenesis. The results showed that hydrogen is the direct electron donor for PCE reduction.

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Expression of reductive dehalogenase genes in Dehalococcoides ethenogenes strain 195 growing on tetrachloroethene, trichloroethene, or 2,3-dischlorophenol

Article Abstract:

Reverse transcriptase quantitative PCR during growth and reductive dechlorination of tetrachloroethene (PCE), trichloroethene (TCE) or 2,3-dichlorophenol (2,3-DCP) is used to investigate reductive dehalogenase (RD) gene transcript levels in Dehalococcoides ethenogenes strain 195. It shows chlorophenols naturally occur in soils and are good candidates for the original substrates for PceA.

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