Characterization of a large, stable, high-copy-number Streptomyces plasmid that requires stability and transfer functions for heterologous polyketide overproduction

Article Abstract:

A mechanism by which SCP2 derived plasmid achieves increased levels of metabolite production was identified to examine the 45-bp deletion mutation in the plasmid replication origin increased plasmid copy number. It was found that 45-bp deletion abolished binding of a plasmid replication protein to the plasmid DNA, supporting an iteron model for plasmid replication.

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Rapid engineering of the geldanamycin biosynthesis pathway by red/ET recombination and gene complementation

Article Abstract:

The use of Red/ET recombination to generate a large number of shuttle plasmids from a diverse library of chimeric domains for directed evolution of the pikromycin polyketide synthase (PKS) is reported. Red/ET recombination technology is adopted for generating new polyketide as it overcame some of the limitations of traditional approaches for PKS engineering.

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Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin obtained by gene sequencing and disruption

Article Abstract:

The cloning and characterization of a major portion of the herbimycin (hbm) biosynthetic gene cluster containing the polyketide synthase (PKS) genes and some other genes involved in post-PKS modifications are described. The results show a novel geldanamycin analog, KOS-1806 which was isolated and characterized.

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