Article Abstract:
Pseudomonas aeruginosa can reactivate UV-damaged bacteriophages by dark repair more efficiently when infected during the stationary phase of growth or during starvation. The extent of reactivation also depended on the type of bacteriophage.
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Article Abstract:
Two bacteriophage collections' abilities to form plaques on multiple bacterial host species were examined. Nine of the 10 phages analyzed were found to be broad-host-range bacteriophages. Findings demonstrated that a multiple-host environment protocol may be more effective for the isolation of bacteriophages by avoiding the selection bias inherent in single-host methods. Transduction was generated by at least two of the broad-host-range bacteriophages.
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Article Abstract:
The lytic virus of Pseudomonas aeruginosa, UNL-1, was observed to express a novel inducible DNA damage reactivation activity in UV-A-irradiated P. aeruginosa host cells. The expression of bacteriophage reactivation was quantified in hosts exposed to either UV-C or UV-A radiation. Around 13-fold induction was observed in UV-A-irradiated host cells. When host cells were exposed to sunlight, reactivation of damaged UNL-1 virus increased eightfold. Findings suggest that a combination of host and virus DNA repair processes contributes to replication of aquatic bacterial viruses.
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