Characterization of Nocardia asteroides isolates from different ecological habitats on the basis of repetitive extragenic palindromic-PCR fingerprinting

Article Abstract:

Thirteen isolates of Nacardia asteroides from both soils and aquatic samples, together with a type strain and two known clinical isolates of this species are characterized by repetitive extragenic palindromic-PCR fingerprinting with the BOX-AIR primer. The resulting DNA fingerprint patterns proved to be strain specific, and cluster analysis distinguished the soil isolates, the aquatic isolates, and the known strains as being in separate groups.

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Fingerprinting of Bacillus thuringiensis type strains and isolates by using Bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR analysis

Article Abstract:

Repetitive extragenic palindromic sequence-based PCR analysis (Rep-PCR) fingerprinting is used to analyze a total of 119 Bacillus thuringiensis strains (83 types strains and 26 native isolates), as well as five B. cereus group species. The B. cereus group-specific Bc-Rep-PCR fingerprinting technique is highly discriminative, fast, easy and able to identify B. thuringiensis serotypes, including nonflagellar and self-agglutinated strains.

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Methods to increase fidelity of repetitive extragenic palindromic PCR fingerprint-based bacterial source tracking efforts

Article Abstract:

The similarity of coefficient and statistical method to use and to produce the highest rate of correct assignment (RCA) in repetitive extragenic palindromic PCR-based bacterial source tracking is determined. It is found that after applying the quality factor threshold, it resulted in 22% to 32% improvement in the overall RCA (ORCA), which also depends on the fingerprinting technique used.

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