Article Abstract:
The amino acid sequence and genetic expression of the LexA protein of Mycobacterium tuberculosis was analyzed to determine the site of LexA binding in the mycobacteria. Analysis of the amino acid sequence of the LexA protein of Mycobacterium tuberculosis indicated the ability of the protein to bind and recognize a Cheo box motif that is similar to the upstream region of Bacillus subtilis SOS-inducible genes. Furthermore, the pAJB303 lacZ fusion construct exhibited the highest beta-galactosidase activity.
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Article Abstract:
The mechanisms involved in the regulation of expression of the acetamidase were analyzed. Promoter-probe shuttle vectors with a chloramphenicol acetyltransferase reporter gene was used to locate the promoter. It was found that the promoter region for the acetamidase-encoding gene lies in 1.5 kb upstream of the acetamidase gene. The nucleotide sequence of this region was identified and a novel open reading frame with homology to genes encoding regulatory proteins in other bacteria was located.
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Article Abstract:
A recombinant assay was developed and employed to optimize conditions for homologous recombination (HR) in Mycobacterium smegmatis. Treatment of competent cells with UV, hydrogen peroxide or mitomycin C failed to enhance the frequency of HR. However, treatment of the DNA with alkali or UV increased recombination frequency, while boiling did not. The introduction of ss phagemid DNA raised the level of HR and eliminated illegitimate recombination.
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