Examination of recovery in vitro and in vivo of nonculturable Escherichia coli O157:H7

Article Abstract:

Research has been conducted on the Escherichia coli O157:H7 strains. The recovery and pathogenicity of these strains stressed experimentally by long-term storage in water and chlorine exposure have been investigated, and the results are discussed.

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Characterization of Escherichia coli O157:H7 from downer and healthy dairy cattle in the upper midwest region of the United States

Article Abstract:

The authors have investigated Escherichia coli O157:H7. The results have demonstrated the prevalence of O157:H7 in fecal and tissue samples from the colons of downer dairy cattle than in those from healthy dairy cattle.

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Export of virulence genes and shiga toxin by membrane vesicles of Escherichia coli O157:H7

Article Abstract:

Membrane vesicles released by Escherichia coli O157:H7 contain DNA encoding the virulence genes eae, stx1 and stx2, as well as uidA, which encodes for beta-galactosidase, while immunoblotting data show that Shiga toxins 1 and 2 are contained within vesicles. This was gleaned from a study that sought to determine whether E coli O157:H7 produces vesicles under normal growth conditions and whether these contain Shiga toxins and DNA and transfer virulent genes. Results indicate that these vesicles play a role in the export of toxic and genetic material. They can facilitate the transfer of genetic material to other enteric organisms and may serve to disseminate toxic material directly to host cells or to bacterial cells.

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