Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase

Article Abstract:

Results show that 4-hydroxyphenylacetate 3-hydroxylase exhibits sequence similarities to several proteins that are implicated in the biosynthesis and biodegradation processes in bacteria and archaea. Data also point out that the enzyme uses as a substrate the reduced flavin adenine dinucleotide .

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Cloning, sequencing, and characterization of a gene cluster involved in EDTA degradation from the bacterium BNC1

Article Abstract:

Researchers report the isolation of an eight-gene cluster from the bacterium BNX1 that encodes some of the enzymes that degrade EDTA. Two of the gene products were identified as a reduced flavin mononucleotide-utilizing monooxygenase and an NADH:flavin mononucleotide oxidoreductase. The others include a transport system, a regulatory gene, and an iminodiacetate oxidase.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Identification, purification, and characterization of iminodiacetate oxidase from the EDTA-degrading bacterium BNC1

Article Abstract:

Researchers have isolated and characterized an enzyme from the EDTA-degrading bacterium BNC1. Iminodiacetate oxidase appears to be the second enzyme in the pathway for degrading both EDTA and NTA.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: