Article Abstract:
The cryptic plasmid, pMBB1, from Enterococcus faecium 226 contains a stable replicon which has some resemblance to the lactococcal plasmid pCI305. Sequence analysis of the plasmid indicates an open reading frame of 822 bp that codes for a 32-kDa protein. Restriction mapping analysis shows that pMBB1 is around 2.85 kb. The pMMB1 plasmid probably belongs to the same family as pCI305 as there are no single-stranded intermediates. The replicon can be used to develop a stable cloning vector that has the ability to replicate in many lactic acid bacteria.
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Article Abstract:
A study was conducted to investigate the genomic relationships between Entorococcus faecium strains from different sources and with varied levels of antibiotic resistance. Restriction endonuclease analysis was used to evaluate total chromosomal DNA of 47 strains. Results indicate that strain clusters can be formed according to host. The major groups of Entorococcus faecium strains were categorized under chicken, pork and human. It was also observed that strains from animals do not group with those of human's.
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Article Abstract:
The effect of virginiamycin exposure on antimicrobial resistance in Enterococcus faecium recovered from treated broilers is evaluated. In an experimental setting using broiler chickens, continuous virginiamycin exposure was required to maintain a stable streptogramin-resistant population of E. faecium in the animals.
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