Article Abstract:
The promoter of the Paracoccus denitrificans nitric oxide (NO) reductase operon (norCBQDEF) has been studied using primer extension and deletion analysis. Two transcription-start sites were seen, the major one active only in cultures grown anaerobically, with nitrate, and is 45.5 bp downstream of the putative NNR-binding site, so this is a class II factor-dependent promoter. NNR is the sole regulatory protein with a significant role in regulating expression of the NO reductase. The NNR-binding site has been confirmed.
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Article Abstract:
Strong expression signals of Mycobacterium tuberculosis have been isolated using the natural fluorescence of the Aequoria victoria green fluorescent protein. Two of the promoters corresponded to proteins thought to be involved in magnesium and calcium utilization, very important for cell physiology and thus controlled by strong transctiption signals. The strength of the expression signals could be used for overexpression of foreign genes in mycobacteria to help with vaccine vector development and protein purification. Fluorescence-activated cell sorting (FACS) has been shown to offer a sensitive, efficient means to measure and select strong mycobacterial expression signals.
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Article Abstract:
Research shows that the expression of the nap gene, encoding the periplasmic nitrate reductase, is influenced by substrates and their reduction state. Data indicate that the highest level of expression and enzyme activity occurred in the order of butyrate greater than acetate greater than succinate.
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