Article Abstract:
The changes in cellular states of the marine bacterium Delaya aquamarina under starvation conditions were examined using different fluorescent dyes and techniques to characterize its heterogeneity and the changes of its physiological states. The experiments were conducted on artificial seawater microcosms at two temperature representative of the temperature similar to the Mediterranean. Results reveal that D. aquamarina has greater maintenance of culturability at lowest temperature. However, culturability is better preserved at lower temperatures for nonmarine bacteria under starvation.
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Article Abstract:
Bacteria adapt to low temperature conditions on long-term incubations. A study of the ability of bacteria to reduce sulfate in two marine sediments in Denmark and Antarctica indicates that the psychrotrophic bacteria grow optimally between 18 and 19 degrees celsius during short-term incubations. Incubations for a week reduce the optimum temperature to 12.5 degrees celsius. The psychrotrophic strains of bacteria reduce sulfate optimally at 28 degrees celsius in short-term incubations. The psychrophilic strains have an optimum growth under a temperature between 30 to 35 degrees celsius.
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Article Abstract:
The 4'-6-diamidino-2-phenylindole staining procedure and the most-probable-number (MNP) counts are appropriate methods for the determination of bacterial counts in filtered seawater cultures. The techniques indicate that the superficial and intermediate water masses have a high and a variable population of living bacteria. The MNP viable counts correlate well with the total counts but are more time-consuming and expensive. The traditional CFU method is able to determine only 0.0001 to 0.2% of the total bacteria.
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