Article Abstract:
The pattern of Pythium ultimum colonization by Trichoderma harzianum was analyzed to determine the biological events that mediate the interaction between the phytopathogenic pathogen and the soil microorganism. The colonization of Trichoderma harzianum by Pythium ultimatum was characterized by the initial recognition of the soil microorganism to the Pythium hyphae. The pathogen weakens the cell wall of the host by producing beta-1,3-glucanases for cell wall penetration. Furthermore, the metabolism of the host cell was altered by the synthesis of antibiotics and cellulases.
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Article Abstract:
Manipulations were done on the ech42 gene of Trichoderma harzianum to study the role of endochitinase Ech42 in mycoparasitism. The 42-kDa Ech42 can hydrolyze Botrytis cinerea cell walls in vitro and stop spore germination. Several transgenic T. harzianum strains with multiple copies of ech42 and the corresponding gene disruptants have been created. Results indicated that T. harzianum secretes ech42 in the presence of a potential host and is not essential for effective plant control.
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Article Abstract:
Conclusive evidence for the induction of a systematic response against regular leaf spot of cucumber following application of T. asperellum to the root system is presented. The results suggest that similar beneficial rhizobacteria T. asperellum may activate separate metabolic pathways in cucumber that are involved in plant signaling and biosynthesis, eventually leading to the systemic accumulation of phytoalexins.
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