Article Abstract:
Ruminococcus albus when incubated with a combination of cellubiose and glucose, the organism used up the dissacharide. This preference seemed to be related to repression of glucose uptake system in cellubiose-grown cells. Glucose transport kinetics indicated low and high affinity uptake, and high-affinity transport was caused by ATP hydrolysis. Bacterial yield was 38% greater when the organism was grown on cellobiose, and this could be due to constitutive cellobiose phosphorylase activity. Substrate affinities analyzed in carbon-excess continuous culture showed that affinities were 0.97 and 3.16 mm respectively.
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Article Abstract:
The metabolism of nucleic acids by ruminal bacteria was investigated focusing on one nucleic acid degradative product, 2-deoxyribose (2DR). 2DR enrichment cultures of bovine rumen contents showed that the only 2DR-degrading strain present in bovine rumen is Selenomonas ruminantium. This result was confirmed by screening a diverse range of previously characterized ruminal bacteria. The 2DR-degrading microbe was present at only low population densities, indicating that there is a low concentration of free 2DR in the rumen.
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Article Abstract:
The utilization and transport of xylose and arabinose in Selenomonas ruminantium and the effect of glucose and hexose disaccharides on pentose utilization are discussed. Continuous culture studies were used to relate dilution to co-utilization and their individual transport activities. The effects of substrate preferences on utilization patterns and growth rates demonstrate the complexity of rumen bacterial growth.
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