Article Abstract:
The transport of cellobiose in mixed ruminal bacteria harvested from a holstein cow fed with Italian ryegrass hay was determined in the presence of nojirimycin-1-sulfate, which almost inhibited cellobiase activity. Extracellular and cell-associated cellobiases were detected in the rumen. The proportion of cellobiose that was directly transported before it was extracellularly degraded into glucose increased as the cellobiose concentration decreased. Cellobiose was incorporated into the cells mainly through the phosphoenolpyruvate phosphotransferase system.
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Article Abstract:
An investigation of the reasons for the inability of mixed ruminal bacteria to degrade all of the peptides from enzymatic digests of casein and gelatin despite long hours of incubation is discussed. Ninhydrin procedure was used to estimate peptide and amino acid utilization and high pressure liquid chromatography assayed amino acid composition of the residual peptides and freeamino acids. It was found that the presence of proline affected the in vitro degradation of the peptides.
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Article Abstract:
The DnaB intein function was altered to develop a new intein based method for generating the Staphylococcus aureus autoinducing peptides (S. aureus AIPs) signals. Results conclude that the method is simple and scalable and helps biological efforts by producing S. aureus AIPs.
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