Article Abstract:
The virally encoded K28 killer toxin of Saccharomyces cerevisiae kills sensitive cells by a receptor-mediated process. DNA synthesis is prevented fast, cell viability is lost more slowly and cells eventually arrest. Cytoplasmic microtubules seem to be normal, and there is no spindle. Arrest of a sensitive haploid yeast strain by alpha-factor at START gave complete protection for at least four hrs. against a toxin concentration that killed non-arrested cells at the rate of one log each 2.5 hrs.
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Article Abstract:
A study was conducted to analyze whether mutant Shiga-like toxin (SLT) undergo proteolytic cleavage at a second distinct site. A recombinant SLT-1 mutant that does not undergo any proteolytic cleavage during cell entry was utilized to carry out the analysis. The cytotoxicities of purified recombinant toxins to cultured mammalian cells were compared against each other. Results indicated that SLT is not significantly attached to the cell-binding peptides of the B-subunit pentamer.
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Article Abstract:
Investigation of killer yeast strains showed that spore-forming ascomycetous yeast of the Pichia and Williopsis genera displays the broadest range of activity against sensitive strains of Candida spp. and Saccharate cerevisiae. Williopsis mrakii exhibited strong anti-Candida activity against isolated clinical specimens. This toxin has the potential as a antimycotic agent in combatting strains of Candida.
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