Article Abstract:
The protein cyclin-dependent kinase 2 (Cdk2) begins the process of mitosis by regulating the complexation of Cdc2 protein kinase with B cyclins. When Cdk2 was inhibited by a specific inhibitor, mitosis is halted and a concentration of Cdc2-cyclin b complexes is observed. This effect was readily reversed by either the addition of Cdk2-cyclin E or the addition of cyclin B. This pathway serves as a controlling point for preventing early mitosis while DNA synthesis is still occurring in the cell.
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Article Abstract:
The yeast cyclin-dependent kinase inhibitor p25(super rum1) controls the cell cycle by suppressing the activity of the p34(super cdc2)/p56(super cdc13) mitotic kinase. The protein, encoded by the rum gene, inhibits mitosis of cells present in the G1 and G2/M phase of the cell cycle. Premature mitosis of cells in the G1 phase is lethal. Overexpression of the rum gene causes repeated DNA replication by removing the block on the S phase. The protein also determines the length of the G1 phase.
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Article Abstract:
Studies have shown how the cell cycle checkpoint control that prevents mitosis when DNA is damaged, interacts with cell cycle regulators to stop the onset of mitosis. It is suggested that there is a common molecular mechanism underlying such control. It is likely that fission yeast is a suitable model system for further characterization of the checkpoint pathway and the development of suitable assays and screening procedures.
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