Catabolism of D-glucose by Pseudomonas putida U occurs via extracellular transformation into D-gluconic acid and induction of a specific gluconate transport system

Article Abstract:

The metabolic mechanisms that mediate the transformation of D-glucose to D-gluconic acid by Pseudomonas putida U were analyzed in a chemically defined medium containing phenylacetic acid (PA) as the sole carbon source. Analysis of the degradation of D-glucose to D-gluconic acid by Pseudomonas putida U indicated the presence of a dehydrogenase and an D-gluconic-acid-specific transport system that mediated the oxidation of D-glucose. Furthermore, the microbe utilized the pentose phosphate pathway for the catabolism of D-glucose.

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Purification of Pseudomonas putida acyl coenzyme a ligase active with a range of aliphatic and aromatic substrates

Article Abstract:

An acyl coenzyme A synthetase (ACoAS) was purified from Pseudomonas putida and compared with other varieties of ACoAS. Sodium dodecyl sulfate- polyacrylamide gel electrophoresis was used in the experiment. Molecular masses were estimated. In vitro enzymatic reactions were used to generate antibiotics. The high catabolic potential and the molecular basis of the catabolic behavior of this microbe was demonstrated.

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Strategy for cloning large gene assemblages as illustrated using the phenylacetate and polyhydroxyalkanoate gene clusters

Article Abstract:

A simple method based on a single recombination event is developed to facilitate the cloning of all the genes and regulatory elements included in large genetic assemblages. The entire set of genes belonging to the phenylacetic acid (PhAc; 18-kb) in Pseudomonas putida U were recovered directly from bacterial chromosome and cloned into a plasmid by following this methodology.

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