Carbon monoxide, hydrogen, and formate metabolism during methanogenesis from acetate by thermophilic cultures of Methanosarcina and Methanothrix strains

Article Abstract:

The metabolism of carbon monoxide (CO), hydrogen (H2) and formate by thermophilic cultures of Methanosarcina thermophila TM-1 and Methanothrix sp. strain CALS-1 were compared. The results showed that both species had fairly high levels of formate dehydrogenase. M. thermophila accumulated H2 at significantly higher levels compared to Methanothrix sp.. In contrast, Methanothrix sp. accumulated and consumed CO while M. thermophila did not. These results suggest that the function of H2 in M. thermophila is similar to that played by CO in Methanothrix sp..

author: Zinder, S.H., Anguish, T.
Microbial metabolism, Hydrogen, Carbon monoxide, Formic acid

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Interactions between nitrogen fixation and osmoregulation in the methanogenic archaeon Methanosarcina barkeri 227

Article Abstract:

The role of salt on the in vivo and in vitro diazotrophy of various cations and anions of the methanogenic archaeon Methanosarcina barkeri 227 strain has been studied. The osmolyte accumulation in both diazotrophic and ammonium-grown cells have also been investigated to find out if there was a shift towards nonnitrogenous osmolytes under diazotrophic regimes. Results indicate that inhibition of growth was not greater with N2 than with NH4+. Cells grown with N2 and with NH4+ produced equal amounts of alpha-glutamate at low salt concentrations.

author: Zinder, S.H., Drabban, A.D., Orcutt, E.N.
Fluid-electrolyte balance, Nitrogen, Osmoregulation, Nitrogen fixation, Salt, Salt (Food)

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Comparative proteomics of Dehalococcoides spp. reveals strain-specific peptides associated with activity

Article Abstract:

The species of Dehalococcoides contain enzymes reductive dehalogenases (RDases) that anaerobically reduce the halogenated compounds. The proteins of RDase and homologous proteins like PceA, TceA and VcrA are identified and reported in the proteomics data.

author: Morris, R.M., Zinder, S.H., Fung, J.M., Rahm, B.G., Zhang, S., Freedman, D.L., Richardson, R.E.
Science & research, Genetic aspects, Nucleotide sequence, Base sequence, Anaerobic bacteria, Bacterial proteins

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subjects list: Research, Physiological aspects, Methanobacteriaceae, Methanogens
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