Article Abstract:
Molecular genetic analysis of group 1 mutants of the carbapenem antibiotic-producing Erwinia carotovora GS101 involves the isolation of a complementary cosmid cWU142. Sequence analysis of a cWU142 subclone reveals that the carR gene codes for a homologue of the Vibrio fischeri LuxR protein. The carR gene serves as a hot spot target for a Tn5 element transposition, leading to the formation of carR mutants at a high frequency. Genes that code for several metabolic functions such a plasmid conjugation, bioluminescence and cell division are transcriptionally induced by the carR protein, which depends on N-acyl homo-serine lactones for its function.
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Article Abstract:
The isolation and characterization of the first generalized transducing phage for Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043 was reported. It was found that the phage (phiM1) infects a large spectrum of Eca strains which could be useful for the general genetic analysis of many Eca isolates and for the transductional transfer of selectable markers between strains.
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Article Abstract:
A carbapen-2-em-3-carboxylic acid (CarR) protein with homology to the LuxR family of transcriptional regulators is encoded to isolate and identify a Serratia marcescens carR gene. Test results show that this protein is capable of activating carbapenem synthesis in the said bacteria. Moreover, CarR can release a heterologous Erwinia carotovora host for carbapenem production.
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