Article Abstract:
A study was conducted to compare the effects of known polymerase chain reaction (PCR)-inhibiting samples on nine thermostable DNA polymerases. Samples of blood, cheese, feces and meat as well as various ions were added to PCR mixtures containing various thermostable DNA polymerases. Th eDNA polymerase from Thermotoga maritima was found to be the most susceptible to PCR inhibitors present in cheese, feces and meat samples. Results suggested that the PCR-inhibiting effect of various components in biological samples can be eliminated to some extent by the use of appropriate DNA polymerase.
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Article Abstract:
A research was conducted to study the level of occurrence of chimeric sequences after polymerase chain reaction (PCR) using a model in which 16S rRNA genes were amplified from mixed bacterial genomes. The DNASTAR program was utilized to examine the multiple alignment of sequences and sequence similarities. Results showed that the use of PCR in the study of microbial diversity revealed the existence of several novel organisms that cannot be isolated by culture-dependent techniques.
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Article Abstract:
Research was conducted to examine the presence of maltose and maltotriose transporter gene sequences in brewer's yeast. To examine the alleles of maltose and maltotriose transporter genes, 30 brewing strains of yeast, which include five ale strains and 25 lager strains, were studied. Results revealed the presence of alleles of known alpha-glucoside transporter genes. Findings also demonstrated that related sequences map to other chromosomes in some of the genomes examined.
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