CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint

Article Abstract:

Cells in the process of meiotic development must have C1b-dependent cyclin-dependent protein kinase (CDK) activity for replication of DNA. In the budding yeast the B-type cyclins C1b5 and C1b6 are the main activators of the S phase function of CDK Cdc28. Diploid c1b5/c1b5 clb6/c1b6 mutants cannot carry out premeiotic DNA replication. A DNA replication checkpoint that depends on the ATM homolog MEC1 operates in wild-type cells in meiosis. It can be invoked as a response to inhibition of DNA synthesis. CLB5 and CLB6 are necessary for premeiotic DNA replication and activation of the meiotic S/M checkpoint.

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S-phase specific activation of Cds1 kinase defines a subpathway of the checkpoint response in Schizosaccharomyces pombe

Article Abstract:

Activation of Cds1 kinase that is S-phase-specific gives definition of a subpathway of the checkpoint response in Schizosaccharomyces pombe. Cds1 kinase is necessary to slow S phase where there are of DNA-damaging agents. Cds1 is not necessary for the mitotic-arrest checkpoints; it defines an S-phase specific subpathway of the checkpoint response. That is different from what happens with the Saccharomyces cerevisiae counterpart, Rad53. It seems that an intrinsic mechanism linking S phase and mitosis may work in a way that is independent of known checkpoint proteins.

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The DNA-dependent protein kinase

Article Abstract:

The DNA-dependent protein kinase (DNA-PK), the physiological targets of which are still not precisely identified, is activated when associated with DNA and DNA damage. DNA-PK is critical in DNA DSB repair. DNA-PK parts have roles in other ways, among them controlling telomeric integrity and chromatin structure. Identification of novel double-strand break (DSB) repair components working with DNA-PK will be important. More will be found out about functionally regulated kinases and how they deal with genomic damage as more is found out about DNA-PK.

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