Biosynthesis of yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host

Article Abstract:

The successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Escherichia coli as a heterologous host, is reported. The success has implications for basic and applied studies on Ybt biosynthesis and also, more generally, for future production of polyketide, nonribosomal peptide, and mixed polyketide-nonribosomal peptide natural products using Escherichia coli.

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Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus

Article Abstract:

Degenerate PCR primers targeted to conserved sequence motifs of known nonribosomal peptide synthetase (NRPS) genes were used to amplify gene fragments from a cereulide-producing Bacillus cereus strain. It was concluded that the valine-activating module was highly conserved when sequences form nine emetic B. cereus strains isolated from diverse geographical locations were compared.

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In vivo production of artificial nonribosomal peptide products in heterologous host escherichia coli

Article Abstract:

Description of fermentation of cyclic dipeptide as a prototype for the exploitation of Escherichia coli is given in the study. The use of artificial nonribosomal peptide synthetases is also explained.

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