Biosynthesis of fluorinated secondary metabolites by Streptomyces cattleya

Article Abstract:

The growth of Streptomyces cattleya was slow in cultures containing fluorine, and when maximum growth had taken place fluoroacetate and 4-fluorothreonine were formed by the cells. The formation of these compounds was maximum when the pH is 6.0 and no interconversion of these compounds was observed. Out of all the compounds tested, only (U-14C) glycolate increased the amount of fluoroacetate as it is probably used as a substrate in the biosynthesis of fluoroacetate. Older resting cells contain more fluoroacetate than 4-fluorothreonine.

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Comparison of the efficacies of chloromethane, methionine, and S-adenosylmethionine as methyl precursors in the biosynthesis of veratryl alcohol and related compounds in Phanerochaete chrysosporium

Article Abstract:

Phanerochaete chrysosporium convert L-methionine to chloromethane and utilize it as a methyl precursor in the biosynthesis of veratryl alcohol, without using S-adenosylmethionine as an intermediate. Chloromethane and L-methionine facilitate, while S-adenosylmethionine inhibits the initiation of veratryl alcohol biosynthesis. The biosynthesis involves para-hydroxylation, 4-hydrobenzoic acid methylation, 4-methoxybenzoic acid meta-hydroxylation to produce isovanillic acid, and isovanillic acid methylation to produce veratric acid.

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Evidence for the existence of independent chloromethane- and S-adenosylmethionine-utilizing systems for methylation in Phanerochaete chrysosporium

Article Abstract:

The utilization of C2H3Cl and S-adenosyl-L-(methyl-2H3)methionine as methyl donors in methylation of acetovanillone in whole cells and in cell extracts of Phanerochaete chrysosporium at different stages of growth was reported. Methylation of acetovanillone by the labelled methyl donors was examined in the presence and absence of S-adenosylhomocysteine. The results show the existence of two biochemically distinct systems for O methylation of acetovanillone utilizing SAM and CH3Cl, respectively, as the methyl donor.

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