Article Abstract:
A procedure was developed for the bioremediation of dinoseb-contaminated soils which involved flooding of contaminated soils with phosphate buffer and supplementation with a starch source a by-product of 'centrifuge cake' from a potato processing plant. These enhanced the establishment of anaerobic conditions which was necessary for the inoculum to degrade dinoseb. Tests on contaminated soils showed that the enhancement procedures may be sufficient in less contaminated soils because of the presence of native degrading microorganisms. In more highly contaminated soils, the use of an acclimated inoculum reduces the degradation time significanlty.
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Article Abstract:
The optimum conditions for bioremediation of munition compound-contaminated soils were established. The procedure called for the establishment of anaerobic conditions by phosphate buffer flooding of contaminated soils followed by the addition of potato starch. Optimum pH was set at low to neutral at a temperature range of 20 to 37 degrees celsius. Addition of a nitrogen source also contributed to the remediation of soils. Application of all these conditions resulted in a highly increased rate of the reductive phase of 2,4, 6-trinitrotoluene degradation.
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Article Abstract:
High-performance liquid chromatography is used to degrade 2-sec-butyl-4,6-dinitrophenol (dinoseb) with a strain of Clostridium bifermentans acting as a biocatalyst. The degradation process is performed below the limit of detection with no accumulation of aromatic intermediates. Results show that C. bifermentans can easily mineralize the anaerobic degradation products of dinoseb.
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