Biogeography, host specificity, and molecular phylogeny of the basidiomycetous yeast Phaffia rhodozyma and its sexual form, Xanthophyllomyces dendrorhous

Article Abstract:

A study was conducted to provide better insight into biogeography, ecology and molecular phylogeny of Phaffia rhodozyma, a basidiomycetous yeast found in tree exudates in the Northern Hemisphere at high altitudes and latitudes. The comparison of internal transcribed spacer (ITS)-based phylogenies of Phaffia rhodozyma and its tree host suggest that different yeast lineages colonize different trees, also provide an explanation for the phylogenetic distance observed between the type strains of Phaffia rhodozyma and Xanthophyllomyces dendrorphous.

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Engineering of chimeric class II polyhydroxyalkanoate synthases

Article Abstract:

A novel combinatorial method of molecular breeding to generate libraries of evolved mutants with enhanced in vivo activities toward polyhydroxyalkanoate (PHA) biosynthesis is discussed. The genetic engineering scheme will be a very useful approach for improving the catalytic activities of other types of desirable enzymes as well.

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Alternative splicing of transcripts from ctrI and ctrYB genes of Xanthophyllomyces dendrorhous

Article Abstract:

The authors have studied Xanthophyllomyces dendrorhous strain UCD 67-385. They have cloned the unexpected cDNA obtained from the strain's ctrI and ctrYB and ctrYB genes' RNAs could be spliced in alternative splice sites.

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