Article Abstract:
Clostridium botulinum type E strains isolated from fresh fish and from different types of fishery products and seafood were characterized via pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis. The objective is to examine the biodiversity of type E strains and to evaluate the effects of fish species, processing and geographical origin on the epidemiology of the isolates. Results showed a high genetic variation, which is surmised to be a result of a lack of strong selection factors that would influence the evolution of type E.
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Article Abstract:
An optimized pulsed-field gel electrophoresis (PFGE) protocol is used to study the strains of proteolytic Clostridium botulinum from various origins in order to learn about the biodiversity of proteolytic C. botulinum and evaluate the usefulness of PFGE in the genotyping of this organism. The results show that PFGE is considered to be a useful tool for molecular epidemiological analysis of proteolytic C. botulinum types A and B.
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Article Abstract:
The applicability of amplified fragment length polymorphism (AFLP) analysis in characterization of Clostridium botulinum groups I and II are assessed and the genetic biodiversity of C. botulinum types A, B, E, and F are analyzed. The results show that AFLP is a fast, highly discriminating and reproducible DNA fingerprinting method with excellent typeability that can be also be used for C. botulinum group identification.
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