Bioassay for nisin in milk, processed cheese, salad dressings, canned tomatoes and liquid egg products

Article Abstract:

A sensitive nisin quantification bioassay was constructed based on Lactococus lactis chromosomally encoding the nisin regulatory proteins NisK and NisR and a plasmid with a green fluorescent protein (GFP) variant gfp(Sub uv) gene under the control of the nisin-inducible nisA promoter. This strain, LAC275, was capable of transducing the signal from extracellular nisin into measurable GFP(sub uv) fluorescence through the NisRK signal transduction system.

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Microplate bioassay for nisin in foods, based on nisin-induced green fluorescent protein fluorescence

Article Abstract:

This article describes construction of a plasmid containing nisin regulatory protein and a green fluorescent protein gene that can sense extracellular nisin and transduce the signal into measurable fluorescence. Results indicate that the assay can measure 2.5 and 45 nanogram and 0.9 and 1.0 microgram of nisin per milliliter of culture medium and milk, cheese and salad dressing, respectively.

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A nisin bioassay based on bioluminescence

Article Abstract:

Researches describe an assay for the antibiotic peptide nisin. The assay uses a strain of Lactococcus lactis that detects nisin and creates a bioluminescent signal. The detection limit of this assay is lower than other assays.

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