Article Abstract:
Environmentally safe biopesticides are the subject of much study and of these, bacillus thuringiensis represents a promising one. The CryIVD toxin of Bacillus thuringiensis subsp. israelensis binds to the proteins of 148 kDa in Anopheles stephensi and of 78 kDa in Tipula oleracea, as indicated by the ligand-blotting experiments on dipteran brush border membrane vesicles (BBMV). Liability towards the CryIVD toxin is 10000 times more for larvae of A. stephensi than for T. oleracea. In the BBMV of lepidopteran species Manduca sexta, CryIVD binds to six proteins ranging from 48 to 110 kDa, but CryIVD is not toxic here.
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Article Abstract:
The cryIVD gene of Bacillus thuringiensis was introduced into the cyanobacterium Agmenellum quadruplicatum PR-6 by translational fusion with the PR-6 phycocyaninB gene on a biphasic plasmid. The ligated product was transformed into the cyanobacterium, and found to express the cryIVD gene product at a high level. Larvicidal assays on the mosquito Culex pipiens showed that within six days, all larvae that had ingested the altered cynobacterium were dead.
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Article Abstract:
The insecticidal activities of genes constructed from Eschirichia coli or Bacillus thuringiensis was studied. These interactions were determined by the derivation of different DNA fragments from the 9.4 kb HindIII of pMI which was introduced into a shuttle vector of B. thuringiensis for expression. Electrophoretic and immunoblot analyses were to characterize the resulting proteins. A synergistic interaction was observed.
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