Beta-glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis

Article Abstract:

The chemical composition of the wild-type and mutant Exophiala (Wangiella) dermatitidis cell walls was analyzed via ConA-fluorescein isothiocyanate (FTIR) labelling and light microscopy (LM). ConA-FTIR and LM analysis of the cell walls of the mutant and wild-type pathogenic black yeast indicated the presence of carbohydrates, glycoproteins and melanin. The cell wall of the pathogenic fungi also contained 1,6-beta-glycosylated proteins. Furthermore, melanized wild-type yeast cells exhibited zymolase resistance compared to non-melanized cells which were highly sensitive to the enzyme.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Cell wall perturbation in yeast results in dual phosphorylation of the Slt2/Mpk1 MAP kinase and in an Slt2-mediated increase in FKS2-lacZ expression, glucanase resistance and thermotolerance

Article Abstract:

Damage to the cell wall of yeast activates the MAP kinase Slt2/Mpk1, which in turn increases FKS2-lacZ expression. This caused compensatory changes in the cell wall resulting in resistance to glucanase digestion and heat shock.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Strategies to determine the extent of control exerted by glucose transport on glycolytic flux in the yeast Saccharomyces bayanus

Article Abstract:

Results demonstrate that in Saccharomyces bayanus cells, grown in the presence of glucose, control of glycolytic flux is largely confined to the glucose transport activity across the plasma membrane.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: