Bel-2 antiapoptotic protein mediates verotoxin II-induced cell death: possible association between Bcl-2 and tissue failure by E. coli O157:H7

Article Abstract:

An association may exist between Bcl-2 and tissue failure related to Escherichia coli O157:H7. The molecular machinery of cell-death induction by verotxins (VTs) has been studied. Verotoxin II (VTII) subunit A has a pentameric sequence from the BH1 domain and interacts with mitochondrial Bcl-2 to bring on target cell death that is the result of caspase 3 activation. VTII interacts with Bcl-2 through each BH1 domain pentameric sequence. VTI does not. The VTII/Bcl-2 complex is required for induction of cell death in target cells.

author: Suzuki, Atsuchi, Doi, Hirofumi, Matsuzawa, Fumiko, Aikawa, Si-ichi, Takiuchi, Kyoko, Kawano, Hirokazu, Hayashida, Midori, Ohno, Susumu
Japan, Cell death, Escherichia coli, Heredity, Microbial toxins

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A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts

Article Abstract:

A novel embryonic poly(A)-binding protein has been found to regulate mRNA deadenylation in extracts of Xenopus eggs. It is apparently responsible for stabilization of poly(A) tails. Concentration of the protein, ePAB, is critical in determining the rate of AU-rich element (ARE)-mediated deadenylation. The goal of the study was to identify factors in ARE-dependent deadenylation in Xenopus early development. An in vitro system was developed from activated egg extracts to mimic in vivo ARE-mediated deadenylation.

author: Steitz, Joan A., Voeltz, Gia K., Ongkasuwan, Julina, Standart, Nancy
United Kingdom, Statistical Data Included, Carrier proteins, Transport proteins, Xenopus, Cytogenetics, Adenylic acid, Adenosine monophosphate

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MicroRNAs repress translation of [m.sup.7] Gppp-capped target mRNAs in vitro by inhibiting initiation and promoting deadenylation

Article Abstract:

The article presents findings in the field of research done on investigating the mechanisms by which miRNAs control gene expression.

author: Standart, Nancy, Jackson, Richard J.
Science & research, Gene expression, Genetic research

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subjects list: Research, United States, Physiological aspects, Developmental biology, Embryology, Experimental, Embryological research, Messenger RNA
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