Behavior of Escherichia coli and male-specific bacteriophage in environmentally contaminated bivalve molluscs before and after depuration

Article Abstract:

The differential elimination of Escherichia coli and male-specific (F+) bacteriophage from oysters and mussels by UV depuration does not appear to be affected by the duration of sewage exposure. Furthermore, after 48 hours of depuration, all E.coli was reduced to undetectable levels in all tissues in mussels, whereas 30% of the initial level of F+ bacteriophage remained in the digestive gland. This suggests that longer depurination may allow better elimination of viruses from shellfish.

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Rapid and sensitive detection of noroviruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples

Article Abstract:

The study developed broadly reactive one-step TaqMan reverse transcription (RT)-PCR assays for the detection of genogroup I (GI) and GII noroviruses (NoV) in fecal samples, as well as shellfish samples. These assays provide rapid, sensitive, and reliable detection of NoV and should prove to be useful for routine monitoring of both clinical ad shellfish samples.

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Evaluation of F-specific RNA bacteriophage as a candidate human enteric virus indicator for bivalve molluscan shellfish

Article Abstract:

F-specific RNA bacteriophage can identify oysters contaminated with human enteric viruses. In a study of oysters harvested from four commercial sites, all had low levels of E. coli but often had levels of FRNA bacteriophage that exceeded 1,000 PFU per 100 g.

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