Bacterial growth in the cold: evidence for an enhanced subtrate requirement

Article Abstract:

The influence of substrate concentration and growth temperatures on the growth rates and biovolumes of psychrohilic bacteria isolated from the waters of the Arctic Ocean and Conception, Bay Newfoundland was examined. The cultures were grown with proteose peptone-yeast extract and proline at temperatures of -1.5 to 35 degrees C. Results showed the absence of influence of subtrate concentration on growth rates at 10 degrees and above while increased nutrient requirements and higher generation times for low-nutrient cultures were observed at -1.5 to 0 degrees. In addition, biovolumes were largest in both the highest-nutrient media and at the lowest temperatures.

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Enhanced broth media for selective growth of Vibrio vulnificus

Article Abstract:

Vibrio vulnificus is an invasive organism found in oyster meat. It causes life-threatening diseases in humans even at low levels of concentration. This necessitates the use of enrichment broths to facilitate cultivation for diagnostic purposes. A study was launched to determine the components of the enrichment broth that could enhance the selective growth of the organism. V. vulnificus was found to grow better in a broth mixture of peptone, sodium chloride and cellubiose. Colistin methanesulfonate further increased growth while suppressing non-target bacteria.

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Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia River, its estuary and the adjacent coastal ocean

Article Abstract:

PCR-amplified 16S rRNA genes from particle-attached and free-living bacteria in the Columbia River, its estuary and the adjacent coastal ocean were cloned and 239 partial sequences were determined. A wide diversity was observed at the species level within at least six different bacterial phyla, including most subphyla of the class Proteobacteria. In the estuary, most particle-attached bacterial clones were related to members of the genus Cytophaga or of the alpha, gamma or delta subclass of the class Proteobacteria.

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