Article Abstract:
The Bacillus thuringiensis subsp. aizawai transducing genome phi-HD248 is double-stranded and has a size of 47,150 bp. The restriction endonuclease map derived from the enzymes BamHI, BglII, SalI and SstI suggests a partial circular nature for the phage. The restriction fragments of BamHI, SstI and BglII have other full-length restriction fragments on either side. The terminal positions of BamHI, SstI and BglII fragments in all phi-HD248 DNA molecules are different. The enzyme SalI has a unique cleavage site which is probably useful as a vector for genes in B. thuringiensis.
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Article Abstract:
Research was conducted to examine the molecular characterization of phage B40-8 of Bacteroides fragilis HSP40. Three major proteins out of 15 peptides in the SDS-PAGE profile were chosen for N-terminal sequencing. Immunodetection by electron microscopy provide evidence that major protein (MP)1 and MP3 were structural proteins of the phage head and that MP2 was a constituent of the tail. The structure of the genome and the localization of the major proteins in the genomic restriction map of the phage are described.
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Article Abstract:
A temperate phage, phi16, produced from Corynebacterium glutamicum ATCC 21792, was found to lysogenize its host through site-specific recombination. The phage attachment location, attP, was localized to a 6.5 kb BamHI fragment of the phi16 genome. This fragment also held phi16 integrative functions. The comparison of the nucleotide sequences of attB, attL, attR and attP indicated a common 29 bp sequence, the core sequence. It is found 11 bp downstream of the 3' end of the integrase gene.
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